![]() ![]() At present this disease is observed on leaf, stem and on fruits, and has been responsible for the removing of many orchards in south India. Punicae, considered to be a minor disease, was reported as bacterial leaf spot in the 1950s. Bacterial blight of pomegranate is among the most devastating natural calamity that inflicted huge losses to pomegranate crop productivity especially in. Our data demonstrate that qPCR is more sensitive than other PCR methods along with being reliable for early diagnosis. Bacterial blight of pomegranate, caused by Xanthomonas axonopodis pv. qPCR detected bacterial blight in orchards that did not show any disease symptoms. However, conventional PCR-AGE detected pathogen at the onset of disease symptoms with a detection limit of 10 pg of bacterial DNA. PCR-CE and qPCR were capable of diagnosing bacterial blight 6 to 10 days before symptom appearance, with detection limits of 100 fg and 10 fg of bacterial DNA respectively. Pomegranate, bacterial blight, Xanthomonasaxonopodis pv punicae(XAP), bacteriophages, plant extracts, cow urine, antibacterial activity. punicae) in recent years has become one of the most serious diseases of pomegranate ( Punica granatum L.) in all the major pomegranate states (Maharashtra, Karnataka, Andhra Pradesh and Gujarat) of the country resulting in enormous losses to growers. The secondary spread of bacterium is mainly. PCR coupled with agarose gel electrophoresis (PCR-AGE), PCR coupled with capillary electrophoresis (PCR-CE) and real-time PCR (qPCR) were applied for the early and accurate diagnosis of bacterial blight in pomegranate. Bacterial blight (Xanthomonas axonopodis pv. Bacterial blight of pomegranate-affected leaves, twigs, and fruits are potential sources of primary inoculum. punicae is serious and major disease in pomegranate (Punica granatum L.) cultivation in. ![]() DNA-based disease diagnostics using polymerase chain reaction (PCR) are reliable, precise, accurate and quick. The bacterial blight of pomegranate caused by Xanthomonas axonopodis pv. Symptoms based disease diagnostic methods are labor-intensive, time-consuming and may not detect disease on asymptomatic plants. To our knowledge, this is the first report of bacterial blight on pomegranate caused by X. punicae on pomegranate has been previously reported in India (2), Pakistan (1), and South Africa (4). Precise and early diagnosis of bacterial blight is crucial for active surveillance and effective management of the disease. High incidence of bacterial blight caused by X. Bacterial blight drastically reduces the yield and quality of fruits, which are critical for pomegranate production. punicae is a major disease of pomegranate. Bacterial blight drastically reduces the yield and quality of fruits, which are critical. The pathogen infects a broad range of cultivated varieties irrespective of their growth stage. punicae is a major disease of pomegranate. Damage is caused by the airborne bacterium Xanthomonas axonopodis pv. The increase in day temperature (38.6☌) and afternoon relative humidity of 30.4% along with cloudy weather and intermittent rainfall favored the disease initiation and further spread of the disease.Bacterial blight caused by Xanthomonas axonopodis pv. Bacterial blight caused by Xanthomonas axonopodis pv.
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